CSPs: Soil lipid biomass; fungi and bacteria, 12 CSPs, overview

Usage Rights

This data is Free for public.

Permission is granted to anybody to access, use and publish all open for public data freely. The commercial use of any data is prohibited. The quality and completeness of data cannot be guaranteed. Users employ these data at their own risk. In order to make attribution of use for owners of the data possible, the identifier of ownership of data must be retained with every data record. Users must publicly acknowledge, in conjunction with the use of the data, the data owners. Cite the data as follows: Wu, Y.T., Buscot, F., Gutknecht, J. and Wubet, T. (2009): Soil lipid biomass: fungi and bacteria, 12 CSPs, overview. BEF-China data portal (Accessed through URL http://china.befdata.biow.uni-leipzig.de/datasets/206)

Dataset Abstract

This is the 2008 CSP PLFA data from a subset of 12 CSPs representing the age gradient. Samples were analyzed over the depth profile of each CSP (see detailed methods below) and analyzed for phospholipid fatty acid profiles, which represent both the biomass and broad community structure of the soil microbial community including broad groups of bacteria and fungi. The point of this study was to characterize the microbial community of the CSPs, as well as to determine which factors most influence microbial community dynamics, including forest age and other factors. Soil lipid fractions can be seen as functional components of the soil microbes, such as decomposers, etc. This dataset summarizes the different groups of lipid fractions, fungi, bacteria.

Dataset Design

A subset of 12 CSPs were selected according to an successional age and a diversity gradient; based on the original age classifications which were later changed: young (CSPs 16,17,25,26), medium (CSPs 1,5,8,9) and old (CSPs 2,4,12,13). At each CSP, samples were originally taken by SP6 at 5 cm intervals down to 50 cm, and then combined according to soil horizons. For each depth, samples were taken from all 9 subplots then combined into one pooled sample. Microbial biomass: all lipids less than 20 carbons in length were summed to give the total biomass. Lipids were otherwise used according to specific microbial indicator groups and either the abundance (nmol lipid g dry wt-1) or in relative abundance (abundance divided by total lipid biomass). Data were then analyzed in R using multivariate (NMDS) or univariate (co-variate regressions and ANOVA) methods.

Spatial Extent

The Gutianshan National Nature Reserve (NNR) is located in the western part of Zhejiang Province (29º8'18" – 29º17'29" N, 118º2'14" – 118º11'12" E, Fig. 1). The Gutianshan NNR has an area of approximately 81 km2 and was initially established as a National Forest Reserve in 1975 and became a National Nature Reserve in 2001. The NNR comprises a large portion of broad-leaved forests of advanced successional stages (Hu & Yu 2008), which have not been managed since the beginning of the 1990ies, as well as young successional stages and conifer plantations, mainly of Cunninghamia lanceolata and Pinus massoniana. --- The vegetation is composed of different types of subtropical evergreen and mixed broad-leaved forests (Yu et al. 2001). Most of the stands are secondary forests, evidenced by maximum tree ages of 180 years, by agricultural terraces in almost all plots and by the presence of charcoal in almost all soil profiles. Around the Gutianshan NRR extensive deforestation has occurred during the Great Leap Forward in the 1950s, as in most parts of Southeast China. However, due to prevailing steep slopes, the Gutianshan area was only marginally usable for agricultural activities, and thus an exceptionally intact forest cover has been preserved. --- The climate at Gutianshan NNR is warm and temperate with a short dry season in November and December and with warm summers (Fig. 2). The climatic conditions are characteristic for the subtropics with an annual average temperature of 15.1°C, January minimum temperatures of -6.8°C, July maximum temperatures of 38.1°C and an accumulated temperature sum (≥ 5°C) of 5221.5 degree days.

Published

Yu Ting Wu, Jessica Gutknecht, Karin Nadrowski, Christian Geißler, Peter Kühn, Thomas Scholten, Sabine Both, Alexandra Erfmeier, Martin Böhnke, Helge Bruelheide, Tesfaye Wubet, François Buscot; Relationships between soil microorganisms, plant communities and soil characteristics in Chines subtropical forests. Ecosystems (accepted Februrary 2012). doi:10.1007/s10021-012-9533-3

Temporal Extent

Samples were taken in August and September 2008 from the CSPs, taken to Germany, and analyzed in the laboratory afterward (over the next several months).

Taxonomic Extent

bacterial or fungal communities based on lipid indicators, the fungal to bacterial ratio, or total microbial biomass (lipid biomass). See column descriptions for more detail about individual lipid indicators.

Measurement Circumstances

This data was from the PhD thesis of Yuting Wu, the initial data was generated before Jessica Gutknecht was at the UFZ (with Francois Buscot, Uwe Lange, and Tesfaye Wubet), and all calculations, interpretation, and manuscript writing were performed after Jessica Gutknecht was there. Methods used for analysis changed for all the work performed by SP13, so methods used for this dataset should only be noted for this dataset.

Data Analysis

The columns of CSP name, depth, and horizon name are explanitory variables, the response columns are the lipid biomass, fungal to bacterial ratio, and the individual fatty acid indicators --- note that the age classes analyzed here are not the most current age classes. They were changed, but analysis was already deep underway on the paper at that point. --- for the use in subsequent analysis mind that the number of depths sampled per CSP differs, taking the sum is thus biased as well as taking the mean. Best is to use the upper horizon only.

Data columns available in the raw data part of this dataset

Age
the age class assigned at the time of sampling;
Data group: Successional age of a forest plot
Keywords: age
Values
Medium
Young
Old
CSP
BEF research plot name; Reasearch plots of the Biodiversity - Ecosystem functioning experiment (BEF-China). Comparative Study Plots (CSP) in the Gutianshan Nature Reserve, having a size of 30x30m^2, measured on the ground.
Unit: id
Data group: BEF research plot name
Keywords: CSP
Values
CSP01
CSP02
CSP04
CSP05
CSP08
* Nadrowski, K. also contributed to this column.
depth
depth increment of the soil sampled; see "CSP soil profile description II_soil horizons.xls" authored by SP6
Data group: Soil depth
Keywords: soil depth
Values
0-10cm
20-30cm
10-30cm
0-5cm
10-20cm
* Scholten, T., Kühn, P. also contributed to this column.
horizon
the soil horizon name assigned by SP6 to that horizon in the soil profile description and the samples along soil profile; Helper; known on data portal, see "CSP soil profile description II_soil horizons.xls" authored by SP6
Data group: Soil horizon
Keywords: soil horizon
Values
Ah+Al-Bv
Ah-Bv
Bv
Ah
Ah+Bv
* Scholten, T., Kühn, P. also contributed to this column.
lab_num
laboratory number of laboratory of soil science and geoecology in Tübingen
Data group: Laboratory number of Laboratory of Soil Science and Geoecology in Tübingen
Keywords: soil base
Values
3179
3178
3180
3183
3177
sample_num
field name of the sample
Data group: Helper
Keywords: soil base
Values
CSP01-P-1-1
CSP02-P-1-1
CSP01-P-1-4
CSP01-P-1-3
CSP01-P-1-2
PLFA
total sum of lipids found; ; Wu et al., 2012 (see metadata sheet, doi:10.1007/s10021-012-9533-3) (derived from datagroup)
Unit: nmol g dry soil-1
Data group: Phospholipid fatty acid profiles (PLFA)
Keywords: soil lipids, microbial biomass, PLFA
Values
10.916
12.568
1.1280000000000001
10.57
12.958
Bacteria
sum: (12:0, i13:0, i14:0, 14:0, i15:1, i15:0, a15:0,, 15:0, i16:0, 16:1x9c/12c, 16:1x7c, 16:1x7t, 10Me16:0, i17:0, a17:0, cy17:0, 17:0, 10Me17:0, 18:17c, 18:17t, 18:0, 10Me18:0, and cy19:0); ; Wu et al., 2012 (see metadata sheet, doi:10.1007/s10021-012-9533-3) (derived from datagroup)
Unit: nmol g dry soil-1
Data group: Phospholipid fatty acid profiles (PLFA)
Keywords: soil, bacteria
Values
13.324
11.308
12.038
12.46
1.088
Fungi
sum 18:1 w9c, 18:2 w6,9c; ; Wu et al., 2012 (see metadata sheet, doi:10.1007/s10021-012-9533-3) (derived from datagroup)
Unit: nmol g dry soil-1
Data group: Phospholipid fatty acid profiles (PLFA)
Keywords: soil, fungi
Values
0.04
0.224
0.132
0.24
0.2