CSPs: Substrate Induced Respiration from 12 CSPs, through the soil profile

Usage Rights

This data is Free for public.

Permission is granted to anybody to access, use and publish all open for public data freely. The commercial use of any data is prohibited. The quality and completeness of data cannot be guaranteed. Users employ these data at their own risk. In order to make attribution of use for owners of the data possible, the identifier of ownership of data must be retained with every data record. Users must publicly acknowledge, in conjunction with the use of the data, the data owners. Cite the data as follows: Wu, Y.T., Buscot, F., Gutknecht J. and Wubet, T. (2008): Substrate Induced Respiration from 12 CSPs, through the soil profile. BEF-China data portal (Accessed through URL http://china.befdata.biow.uni-leipzig.de/datasets/209)

Dataset Abstract

This is the 2008 CSP SIR data from a subset of 12 CSPs representing the age gradient. Samples were analyzed over the depth profile of each CSP (see detailed methods below) and analyzed for basal respiration, substrate induced respiration, and the quotient CO2, which represent the heterotrophic microbial biomass and ecophysiological capacity of the microbial community. The point of this study was to characterize the microbial community of the CSPs, as well as to determine which factors most influence microbial community dynamics, including forest age and other factors.

Dataset Design

A subset of 12 CSPs were selected according to an successional age and a diversity gradient; based on the original age classifications which were later changed: young (CSPs 16,17,25,26), medium (CSPs 1,5,8,9) and old (CSPs 2,4,12,13). At each CSP, samples were originally taken by SP6 at 5 cm intervals down to 50 cm, and then combined according to soil horizons. For each depth, samples were taken from all 9 subplots then combined into one pooled sample.

Spatial Extent

The Gutianshan National Nature Reserve (NNR) is located in the western part of Zhejiang Province (29º8'18" – 29º17'29" N, 118º2'14" – 118º11'12" E, Fig. 1). The Gutianshan NNR has an area of approximately 81 km2 and was initially established as a National Forest Reserve in 1975 and became a National Nature Reserve in 2001. The NNR comprises a large portion of broad-leaved forests of advanced successional stages (Hu & Yu 2008), which have not been managed since the beginning of the 1990ies, as well as young successional stages and conifer plantations, mainly of Cunninghamia lanceolata and Pinus massoniana. --- The vegetation is composed of different types of subtropical evergreen and mixed broad-leaved forests (Yu et al. 2001). Most of the stands are secondary forests, evidenced by maximum tree ages of 180 years, by agricultural terraces in almost all plots and by the presence of charcoal in almost all soil profiles. Around the Gutianshan NRR extensive deforestation has occurred during the Great Leap Forward in the 1950s, as in most parts of Southeast China. However, due to prevailing steep slopes, the Gutianshan area was only marginally usable for agricultural activities, and thus an exceptionally intact forest cover has been preserved. --- The climate at Gutianshan NNR is warm and temperate with a short dry season in November and December and with warm summers (Fig. 2). The climatic conditions are characteristic for the subtropics with an annual average temperature of 15.1°C, January minimum temperatures of -6.8°C, July maximum temperatures of 38.1°C and an accumulated temperature sum (≥ 5°C) of 5221.5 degree days.

Published

Yu Ting Wu, Jessica Gutknecht, Karin Nadrowski, Christian Geißler, Peter Kühn, Thomas Scholten, Sabine Both, Alexandra Erfmeier, Martin Böhnke, Helge Bruelheide, Tesfaye Wubet, François Buscot; Relationships between soil microorganisms, plant communities and soil characteristics in Chines subtropical forests. Ecosystems (accepted Februrary 2012). doi:10.1007/s10021-012-9533-3

Temporal Extent

Samples were taken in August and September 2008 from the CSPs, taken to Germany, and analyzed in the laboratory afterward (over the next several months). Cmic was estimated with 25 g oven-dry equivalent of field-moist soil by substrate induced respiration. Before analyzing, frozen samples were completely thawed and adjusted to an appropriate moisture content. All samples were pre-incubated at 20 ˚C for at least 48 h. Later the analysis was also carried out at 20 ˚C. Basal respiration (BR) was first measured for 24 hours without amendment, followed by addition of glucose and talcum to measure substrate induced respiration (Cmic). The CO2 production of each sample was measured with an automatic infrared gas analyzer (Type 225 MK3) in an air flushed incubation device. The CO2 production was then averaged over four internal replicates of each sample. This CO2 production can be used to estimate the microbial biomass and to calculate two microbial ecophysiological indicators, the metabolic quotient (qCO2) and the Cmic/ Corg ratio. The qCO2 was determined as the ratio of BR (μg g soil-1 hr-1 CO2-C) to Cmic (μg g soil-1). The Cmic/ Corg ratio was calculated by dividing Cmic (μg) by percent soil organic carbon (Corg).

Taxonomic Extent

No specific organisms were analyzed here, but this data can represent the total microbial heterotrophic biomass

Data Analysis

The parameters of interest were the basal respiration (BR, soil CO2 production with no ammendments), substrate induced respiration (SIR, soil CO2 production after addition of glucose and talc), and the quotient CO2 (qCO2, the ratio of BR/SIR). Data were then analyzed in R using multivariate (NMDS) or univariate (co-variate regressions and ANOVA) methods. The columns of CSP name, depth, and horizon name are explanitory variables, the response columns are the BR, SIR, and qCO2 --- note that the age classes analyzed here are not the most current age classes. They were changed, but analysis was already deep underway on the paper at that point.

Freeformat files (1)

  • CSP4all_new2.xls (108 KB)
    Updated file including functional diversity measures

Data columns available in the raw data part of this dataset

CSP
BEF research plot name; Reasearch plots of the Biodiversity - Ecosystem functioning experiment (BEF-China). Comparative Study Plots (CSP) in the Gutianshan Nature Reserve, having a size of 30x30m^2, measured on the ground.
Data group: BEF research plot name
Keywords: CSP
Values
CSP05
CSP02
CSP01
CSP08
CSP04
* Nadrowski, K. also contributed to this column.
lab_num
laboratory number of laboratory of soil science and geoecology in Tübingen
Data group: Laboratory number of Laboratory of Soil Science and Geoecology in Tübingen
Keywords: soil base
Values
3183
3177
3178
3180
3179
* Scholten, T., Kühn, P. also contributed to this column.
sample_num
field name of the sampleHelper
Data group: Helper
Keywords: soil base
Values
CSP01-P-1-1
CSP01-P-1-2
CSP01-P-1-3
CSP02-P-1-1
CSP01-P-1-4
depth
depth increment of the soil sampledHelper
Data group: Soil depth
Keywords: soil depth
Values
10-30cm
20-30cm
0-10cm
10-20cm
0-5cm
horizon
the soil horizon name assigned by SP6 to that depth incrementHelper
Data group: Soil horizon
Keywords: soil horizon
Values
Ah+Al-Bv
Ah
Ah-Bv
Ah+Bv
Bv
age class
the age class assigned at the time of samplingHelper
Data group: Successional age of a forest plot
Values
Medium
Old
Young
BR
Basal respiration; CO2 production of the soil samples after 24 hours, with no ammendment
Unit: µg g soil-1 hr-1 CO2-C
Data group: Substrate Induced respiration
Keywords: soil, SIR, respiration
Values
0.285
0.24
0.02
0.26
0.3475
SIR
Substrate Induced respiration (SIR, or Cmic). SIR is defined as the CO2 production with glucose ammendment.
Unit: µg Cmic g soil-1
Data group: Substrate Induced respiration
Keywords: soil, biomass, SIR
Values
101.39
101.9
101.98
101.58
109.75
qCO2
quotient CO2; defined as BR divided by SIR
Unit: ng C µg Cmic-1 hr-1
Data group: Substrate Induced respiration
Keywords: soil, SIR, respiration
Values
12.079599674153382
10.344912117275024
1.047943411055803
13.02747791952895
13.218098627351296