Main Experiment: Intensive sampling of enzyme activities of soil microbes under certain tree species (Site A)

Usage Rights

This data is Free within projects.

Data can be used by any member of the BEF-China project, or to others after consent is giving by SP13 (Francois Buscot and Jessica Gutknecht).

Dataset Abstract

The soil microbial activity data from intensive sampling in site A in 2011. Activities of four hydrolytic enzymes involved in breakdown of cellulose (β-glucosidase), xylan(ß-xylosidase), chitin (N-acetyl-glucosaminidase) and polyphosphate (acid phosphatase) were measured by using methylumbelliferone substrate. Another two oxidative enzymes (phenol oxidase and peroxidase) which related to lignin degradation, their activities were analyzed by using substrate 3,3',5,5'-tetra:methyl-benzidine. The enzyme activities were measured on the plate reader (SynergyTM HT, Biotek) in xingangshan field lab.

Dataset Design

Within each plot, three random trees of each tree species were selected for taking soil samples. For each targeted tree, we used soil core (10cm depth, diameter 6cm) took soils at four directions of the target tree on a distance of around 50cm from the tree trunk. Four soil cores of samples were homogenised and pooled into one sample in a plastic bag which represented the targeted tree. Soil samples were immediately sieved (2mm) in the field and preserved with liquid nitrogen.

Spatial Extent

Site A, Xingangshan

Temporal Extent

Soil samples were taken between October and November in 2011 in site A. Soil samples were freeze dried in the field lab andmeasured in the field lab after sampling campaign.

Data Analysis

Enzyme activity was computed as µmol substrate converted per hour per g dry organic matter of sample

Data columns available in the raw data part of this dataset

tree-tag
t tag of the respective individual in the field
Data group: t or s TAG Main Experiment
Keywords: tree-tag
Values
t103320010508
t103320010706
t103320010410
t103320010806
t103320010810
site
site: Site A or B of the Main Experiment
Data group: Experimental Site
Keywords: Site A
Values
A
plot_code
plot code
Data group: Plot name Main experiment (letter, number code)
Keywords: plot
Values
F21
E33
E34
C32
E31
species richness
species richness level according to the design of the Main Experiment
Data group: Taxonomic biodiversity
Keywords: species richness
Values
2
1
16
8
4
species identity
species identity, i.e. the species name
Data group: Scientific plant species name
Keywords: species identity
Values
Castanea henryi
Castanopsis eyrei
Castanopsis sclerophylla
Cyclobalanopsis glauca
Choerospondias axillaris
b-gluc
enzyme activity of β-glucosidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
100.45478915788388
100.41629999904904
100.10946351592192
100.42022389702312
100.63510106188964
* Gutknecht, J. also contributed to this column.
xyl
enzyme activity of N-acetyl-glucosaminidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
14.710463874037917
100.09155453095504
16.05946347304276
112.87058671643484
106.02443802738321
* Gutknecht, J. also contributed to this column.
ap
enzyme activity of acid phosphatase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
1006.0553295155527
1010.9087610559211
1013.2192745244073
1031.3678280096988
1017.4969523325747
nag
enzyme activity of ß-xylosidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
100.28951261670136
102.34974314648791
100.71931447090824
101.26885854836706
102.50009818170298
* Gutknecht, J. also contributed to this column.