Main Experiment: Intensive sampling of enzyme activities of soil microbes under certain tree species (Site B)

Usage Rights

This data is Free within projects.

Data can be used by any member of the BEF-China project, or to others after consent is giving by SP13 (Francois Buscot and Jessica Gutknecht).

Dataset Abstract

The soil microbial activity data from intensive sampling site B in 2012. Activities of four hydrolytic enzymes involved in breakdown of cellulose (β-glucosidase), xylan(ß-xylosidase), chitin (N-acetyl-glucosaminidase) and polyphosphate (acid phosphatase) were measured by using methylumbelliferone substrate. Another two oxidative enzymes (phenol oxidase and peroxidase) which related to lignin degradation, their activities were analyzed by using substrate 3,3',5,5'-tetra:methyl-benzidine. The enzyme activities were measured on the plate reader (SynergyTM HT, Biotek) in xingangshan field lab.

Dataset Design

Within each plot, three random trees of each tree species were selected for taking soil samples. For each targeted tree, we used soil core (10cm depth, diameter 6cm) took soils at four directions of the target tree on a distance of around 50cm from the tree trunk. Four soil cores of samples were homogenised and pooled into one sample in a plastic bag which represented the targeted tree. Soil samples were immediately sieved (2mm) in the field and preserved with liquid nitrogen.

Spatial Extent

Study site, locations
Site B, Xingangshan

Temporal Extent

Soil samples were taken between October and November in 2012 site B.Soil samples were freeze dried in the field lab and measured in the field lab after sampling campaign.

Taxonomic Extent

Range of organisms or individuals

Data Analysis

Enzyme activity was computed as µmol substrate converted per hour per g dry organic matter of sample

Data columns available in the raw data part of this dataset

tree-tag
t-tag of the tree accoring to the design of the main experiment
Data group: t or s TAG Main Experiment
Keywords: tree-tag
Values
t207280011312
t207280010515
t207280011313
t207280011407
t207280010612
site
site
Data group: Experimental Site
Keywords: Site B
Values
B
plot_code
plot code
Data group: Plot name Main experiment (letter, number code)
Keywords: plot
Values
J29
G28
I25
M22
M24
species richness
species diversity level according to the design of the Main Experiment
Data group: Taxonomic biodiversity
Keywords: species richness
Values
4
2
1
8
16
species identity
species taxonomic name
Data group: Scientific plant species name
Keywords: species identity
Values
Betula luminifera
Alniphyllum fortunei
Celtis biondii
Castanopsis fargesii
Ailanthus altissima
b-gluc
enzyme activity of β-glucosidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
103.03022971763303
101.34195921516759
100.87729923751998
100.00728667220557
101.71791280420491
* Gutknecht, J. also contributed to this column.
xyl
enzyme activity of N-acetyl-glucosaminidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
105.9016526421704
100.26382393722967
109.26473122803856
105.5149472790449
105.65974597873277
* Gutknecht, J. also contributed to this column.
ap
enzyme activity of acid phosphatase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
1036.6747588570338
1032.043545903418
1044.3318038666475
1019.9575135485159
1016.4262886272172
nag
enzyme activity of ß-xylosidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
100.13086424826464
107.1469261524294
10.857245056713511
10.3224007656419
102.80775671991486
* Gutknecht, J. also contributed to this column.