NILEx tracer (soil): microbial enzyme activity

Usage Rights

This data is Free within projects.

Data can be used by any member of the BEF-China project, or to others after consent is giving by SP13 (Francois Buscot and Jessica Gutknecht).

Dataset Abstract

The soil microbial enzyme activity data from tracer NILEx 2012, data include all the tracer nilex time points.

Dataset Design

Sampling description (e.g. sampling design)
Sampling process was cooperating with leaf litter decomposition group. The soil beneath the litter laid was taken by soil core (0-10cm soil depth) directly after the leaf litter decomposition group collected the litter residues from the designated mesocosms. Soil were then sieved with 2mm mesh and kept cool before stored in the freezer.

Spatial Extent

Study site, locations
NILEx field site, near site A, Xingangshan

Temporal Extent

Range of times description:Samples were taken in 2012 April, May, July, and Octorber. Analyses were done within one month after the sampling campaign

Taxonomic Extent

Range of organisms or individuals:Activities of four hydrolytic enzymes involved in breakdown of cellulose (β-glucosidase), xylan(ß-xylosidase), chitin (N-acetyl-glucosaminidase) and polyphosphate (acid phosphatase) and two oxidative enzymes (phenol oxidase and peroxidase) which related to lignin degradation were measured.

Data Analysis

Data analysis (What were the parameters of interest and how will they be calculated? What are the expected conclusions and how will effect sizes or significances be quantified?) Enzyme activity was computed as µmol substrate converted per hour per g dry organic matter of sample

Data columns available in the raw data part of this dataset

plot_ID
Plot identety code: TracerNIELEx+Blocknumber+Plotnumber+Diversity+Samples in mixture
Data group: Tracer NILEx Plot
Keywords: plot id
Values
T_B1_P07_D1_Schi
T_B1_P03_D1_Querc
T_B1_P05_D1_Sap
T_B1_P10_D2_Querc_Schi
T_B1_P09_D2_Querc_Schi
mesocosm_ID
mesocosm identety code: TracerNIELEx+Timestep+Blocknumber+Plotnumber+Diversity+Samples in mixture
Data group: mescososm id
Keywords: mescososm id
Values
T_T1_B1_P10_D2_Querc_Schi
T_T1_B1_P05_D1_Sap
T_T1_B1_P09_D2_Querc_Schi
T_T1_B1_P03_D1_Querc
T_T1_B1_P07_D1_Schi
timestep
T0=Start of Experiment, T1=12 days, T2=37 days, T3=101 days, T4=171 days
Unit: time
Data group: time point
Keywords: time point
Values
T4
T3
T1
T2
* Leppert (ehem. Seidelmann), K. also contributed to this column.
days
days of decomposition
Unit: day
Data group: time of decomposition
Keywords: day
Values
12
101
37
171
* Leppert (ehem. Seidelmann), K. also contributed to this column.
block
Blocknumber: B1-3
Data group: Tracer NILEx Block
Keywords: block
Values
B1
B2
B3
* Leppert (ehem. Seidelmann), K. also contributed to this column.
plot
plot numbers: not continuous
Data group: Tracer NILEx Plot
Keywords: plot
Values
P05
P03
P04
P07
P06
* Leppert (ehem. Seidelmann), K. also contributed to this column.
diversity
D1, D2, D3 = Monoculture, 2 species mixture, 3 species mixture
Data group: Tracer NILEx Diversity
Keywords: species richness
Values
D2
D3
D1
* Leppert (ehem. Seidelmann), K. also contributed to this column.
composition
species in mixture : Querc = Quercus acutissima, Schi = Schima superba, Sap = Sapindus mukorossi
Data group: Tracer NILEx species composition
Keywords: Composition
Values
Querc_Sap
Sap
Querc_Sap_Schi
Querc
Querc_Schi
* Leppert (ehem. Seidelmann), K. also contributed to this column.
labelled_species
species that was labelled in the mixture
Data group: TracerNILEx Treatment (15N Labeling)
Keywords: labelled species
Values
Sap
Querc
Schi
* Leppert (ehem. Seidelmann), K. also contributed to this column.
mesocosm_position
randomized mesocosm position in plot at the respective timestep
Data group: mesocosom position
Keywords: mesocosom position
Values
1
4
3
2
* Leppert (ehem. Seidelmann), K. also contributed to this column.
b-gluc
enzyme activity of β-glucosidase
Data group: Enzyme activity
Values
105.8719818649319
103.29949820489927
101.16587209704312
107.05849721247245
102.23590813475123
nag
enzyme activity of N-acetyl-glucosaminidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
1001.3296647369866
101.40667937582354
100.8142278622404
106.45332214973823
107.12061065020173
* Gutknecht, J. also contributed to this column.
phos
enzyme activity of acid phosphatase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
1013.4189851108052
1028.8812595673428
1067.0288285125362
1053.4140782856323
1074.2779296199371
* Gutknecht, J. also contributed to this column.
xyl
enzyme activity of ß-xylosidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: hydrolytic enzyme
Values
102.46383618779588
101.665858718099
100.4816311724889
102.42535984128169
103.23330299952981
* Gutknecht, J. also contributed to this column.
po
enzyme activity of peroxidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: oxidative enzyme
Values
105.7092956
101.0425117
108.2530545
104.501494
112.9900498
* Gutknecht, J. also contributed to this column.
per
enzyme activity of Peroxidase
Unit: nmol activity h-1 g soil
Data group: Enzyme activity
Keywords: oxidative enzyme
Values
100.8345643
101.8714332
10.37768595
100.0165062
101.8528386
* Gutknecht, J. also contributed to this column.