CSPs: Taxonomy and representative sequences of wood-inhabiting fungi in highly diverse subtropical forests, all CSPs

Usage Rights

This data is Free for public.

We thank the administration of the Gutianshan National Nature Reserve for granting research permissions. Bernhard Schmid, Keping Ma, Xiaojuan Liu, Yang Bo, Chen Lin and the entire team of BEF-China are gratefully acknowledged for their support.

Permission is granted to anybody to access, use and publish all open for public data freely. The commercial use of any data is prohibited. The quality and completeness of data cannot be guaranteed. Users employ these data at their own risk. In order to make attribution of use for owners of the data possible, the identifier of ownership of data must be retained with every data record. Users must publicly acknowledge, in conjunction with the use of the data, the data owners.

Cite the data as follows:

Wubet, T. and Purahong, W. (2017): CSPs: Taxonomy and representative sequences of wood-inhabiting fungi in highly diverse subtropical forests, all CSPs. (Accessed through URL: http://china.befdata.biow.uni-leipzig.de/datasets/569)

Dataset Abstract

The deadwood mycobiome, also known as wood-inhabiting fungi (WIF), are among the key players in wood decomposition, having a large impact on nutrient cycling in forest soils. However, our knowledge of WIF richness and distribution patterns in different forest biomes is limited. Here, we used pyrotag sequencing of the fungal internal transcribed spacer (ITS2) region to characterize the deadwood mycobiome of two tree species with greatly different wood characteristics (Schima superba and Pinus massoniana) in a Chinese subtropical forest ecosystem. Specifically, we tested (i) the effects of tree species and wood quality properties on WIF OTU richness and community composition; (ii) the role of biotic and abiotic factors in shaping the WIF communities; and (iii) the relationship between WIF OTU richness, community composition and decomposition rates. Due to different wood chemical properties, we hypothesized that the WIF communities derived from the two tree species would be correlated differently with biotic and abiotic factors.
Our results show that deadwood in subtropical forests harbors diverse fungal communities comprising seven ecological functional groups. We found interesting colonization patterns for this subtropical biome, where Resinicium spp. were highly detected in both broadleaved and coniferous deadwood. In addition, the members of Xylariales were frequently found in Schima. The two deadwood species differed significantly in WIF OTU richness (Pinus > Schima) and community composition (P < 0.001). Variations in WIF community composition of both tree species were significantly explained by wood pH and ecological factors (biotic: deadwood species, basal area and abiotic: soil pH), but the WIF communities derived from each tree species correlated differently with abiotic factors. Interestingly, we found that deadwood decomposition rate significantly correlated with WIF communities and negatively correlated with WIF OTU richness in both tree species. We conclude that the pattern of WIF OTU richness and community composition are controlled by multiple interacting biotic and abiotic factors. Overall, our study provides an in-depth picture of the deadwood mycobiome in this subtropical forest. Furthermore, by comparing our results to results from temperate and boreal forests we contribute to a better understanding of patterns of WIF communities across different biomes and geographic locations.

Dataset Design

Here, we used pyrotag sequencing of the fungal internal transcribed spacer (ITS2) region to characterize the deadwood mycobiome of two tree species with greatly different wood characteristics (Schima superba and Pinus massoniana) in a Chinese subtropical forest ecosystem. All CSPs are included.

Spatial Extent

The Gutianshan National Nature Reserve, all CSP

Published

Witoon Purahong*, Katherina A. Pietsch, Guillaume Lentendu, Ricardo Schöps, Helge Bruelheide, Christian Wirth, François Buscot and Tesfaye Wubet. (2017) Characterization of unexplored deadwood mycobiome in highly diverse subtropical forests using culture-independent molecular technique. Frontiers in Microbiology (revised)

Temporal Extent

2 years

Taxonomic Extent

Wood-inhabiting fungi

Data Analysis

Fungal taxonomy of wood inhabiting fungi, representative sequences of all detected fungi

Data columns available in the raw data part of this dataset

Rep_Seq_ID
Representative sequences identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Phylum
Phylum identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Class
Class identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Order
Order identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Family
Family identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Genus
Genus identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Final OTUs
Final OTUs identification of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.
Rep_Seq
Representative sequences of all wood-inhabiting fungi
Unit: no
Data group: Not specified
Alert Sample values are not displayed because this column hasn't been approved yet.